Biological Activities of Dried Garlic, Red Ginseng and Their Mixture

Water extracts were extracted on water bath for 3 hrs at 90oC after 5 times water was added to hot-air
dried garlic (DG) and red ginseng (RG), respectively. Its extracts were dried on rotary evaporator, and then
the mixture samples were prepared from RG extracts added to DG extracts at 5, 10, and 15%. To test biological
activities such as DPPH, nitrite scavenging, tyrosinase, α-glucosidase, human gastric (AGS), and human colon
cancer cell (HT-29) growth, dryness of DG, RG, and mixture samples were diluted 500～10,000 μg/mL adding
deionized water, respectively. DPPH scavenging activities of mixture sample were slightly higher than DG and
RG sample alone. Nitrite scavenging activity was the highest in DG, and the other samples were below 45%.
Inhibition activity of tyrosinase was below 35.0% in tested all samples. Inhibition activity of α-glucosidase was
lower in DG or RG alone, while its activity of mixture sample was increased in proportion to concentration
of RG extracts. Inhibition of AGS cell growth was more effective in mixture samples than DG and RG alone,
while inhibition of HT-29 cell growth was more effective in DG or RG alone than mixture samples.

Key words: hot air dried garlic, antioxidant activity, tyrosinase, α-glucosidase, MTT assay