To investigate the phytochemical componetns and physiological activity of Panax ginseng sprout (PGS) during low-temperature aging, sprouts were aged at 40 and 60℃ for 0.5, 1, 2, 3, and 4 days. The crude saponin content in PGS aged at 60℃ was found to be higher at an early aging time. However, with aging, the content at 40℃, increased rapidly, and become significantly higher in the PGS aged at 60℃. The total content of phenolic compounds gradually increased with aging, with the highest content being 341.3 gallic acid equivalent (GAE) mg/100 g on day 4 at 40℃ and 431.5 GAE mg/100 g on day 3 at 60℃. Among the ginsenosides, the Re, Rb1, and Rg1 contents were higher and the total content was higher at the early aging time in sprouts aged at 60℃, however, there were no significant differences in the contents on day 4. The 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities increased with aging of PGS. The ABTS activities in PGS aged at 40℃ and 60℃ were highest on day 3 and 2, respectively. Thus, the ginsenoside content and physiological activity of the PGS increased throughout the aging process. The optimal aging temperature and time were 3 days at 40℃ and 2 days at 60℃.